Description
CDNR4 cells are a remarkable subset derived from the COMMA-D cell line, a mouse mammary carcinoma model. CDNR4 cells represent a clonal subpopulation of the COMMA-D cell line that has been extensively characterized for their unique properties and functions. Their similarity to mammary stem cells makes them a valuable tool for studying stem cell biology, carcinogenesis, and cell population heterogeneity. These cells were generated by transfection of a kanamycin and neomycin resistance transposon (Tn5 gene) and have since shown fascinating characteristics and properties. One of the most remarkable aspects of CDNR4 cells is their ability to functionally differentiate into preneoplastic and neoplastic phenotypes. The COMMA-D cell line from which they are derived was first characterized for heterogeneity using techniques such as phase contrast microscopy, immunocytochemical staining, DNA content analysis, and assessment of oncogenic potential. Clonal subpopulations of COMMA-D cells were isolated by transfection and selection procedures, and CDNR4 emerged as one of the notable clones. It is important to note that despite their clonal origin, these subpopulations often retained the heterogeneity observed in the COMMA-D parental lineage